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Objective To study the mechanism of Shexiang Baoxin pill in the treatment of atherosclerosis, and to provide a theoretical basis for long-term clinical application. Methods The chemical components and targets of Shexiang Baoxin pill were collected and screened by TCMSP, TCMID, ETCM and BATMAN databases. The targets related to atherosclerosis were collected and screened by DisGeNet, OMIM, TCMSP, DrugBank and DisGeNet. Drug-compound target network and protein-protein interaction network were constructed. Go and KEGG enrichment analysis of Shexiang Baoxin pill in the treatment of atherosclerosis were carried out on MetaScape platform. Results 114 potential therapeutic components of Shexiang Baoxin pill on atherosclerosis were selected, corresponding to 175 targets. The results of network analysis showed that the main active components of Shexiang Baoxin pill were chenodeoxycholic acid, ursodeoxycholic acid, cinnamaldehyde and ginsenoside Rb1. The results of pathway enrichment showed that the anti-atherosclerotic mechanism of Shexiang Baoxin pill was related to the regulation of immunity, inflammation, and metabolism. Conclusion The active components of Shexiang Baoxin pill could act on ALB, INS, AKT1, ACTB, TNF, IL-6 and other targets, regulating multiple pathways to achieve the therapeutic effect on atherosclerosis.
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OBJECTIVE@#To evaluate the therapeutic efficacy of Shexiang Baoxin Pill combined with exercise in heart failure patients with preserved ejection fraction (HFpEF).@*METHODS@#Sixty patients with HFpEF were randomly divided into group A (n=20), receiving Shexiang Baoxin Pill combined with home-based exercise training based on conventional drugs for 12 weeks; group B (n=20), receiving conventional drugs combined with home-based exercise training for 12 weeks; and group C (n=20), receiving conventional drug treatment only. Peak oxygen uptake (peakVO2), anaerobic threshold (AT), 6-min walking test (6MWT), Pittsburgh Sleep Quality Index (PSQI), and SF-36 questionnaire (SF-36) results before and after treatment were compared among groups.@*RESULTS@#After the 12-week intervention, patients in group C showed significant declines in peakVO2, AT, 6MWT, PSQI, and SF-36 compared with pre-treatment (P<0.01), while groups A and B both showed significant improvements in peakVO2, AT, 6MWT, PSQI, and SF-36 results compared with pre-treatment (P<0.01). Compared with group C, patients in groups A and B showed significant improvements in peakVO2, AT, 6MWT, PSQI, and SF-36 (P<0.01). In addition, patients in group A showed more significant improvements in physical function, role-physical, vitality, and mental health scores on the SF-36 questionnaire, and PSQI scores than those in group B (P<0.01).@*CONCLUSIONS@#Exercise training improved exercise tolerance, sleep quality and quality of life (QoL) in patients with HFpEF. Notably, Shexiang Baoxin Pill played an active role in sleep quality and QoL of patients with HFpEF. (The trial was registered in the Chinese Clinical Trial Registry (No. ChiCTR2100054322)).
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Humans , Heart Failure/therapy , Quality of Life , Stroke Volume , ExerciseABSTRACT
OBJECTIVE@#To investigate the effect of Shexiang Baoxin Pill (, SBP) on early hypertensive renal injury in rats and to explore the possible mechanism.@*METHODS@#Twelve-week-old spontaneous hypertensive rats (SHRs) with high-salt diet (dietary containing 8% NaCl) were randomized into the SBP group [40 mg/(kg·d)], losartan potassium group [20 mg/(kg·d)] and saline group by stratified random sampling method, 12 in each group. Blood pressure and urea albumin creatinine ratio were measured. After 10 weeks, the expression levels of serum creatinine (Scr), hypersensitive C-reactive protein (hs-CRP), interleukin (IL)-1 β, IL-6, tumor necrosis factor α (TNF-α), and transforming growth factor β (TGF-β) in serum were assessed. Kidney pathology periodate-schiff staining was performed. Semi-quantitative count of macrophage infiltration was determined by immunochemistry of CD68 staining. Real-time quantitative polymerase chain reaction and Western blot were performed to examine the mRNA and protein expressions of Toll-like receptor 4 (TLR4), nuclear factor κB (NF-κB), monocyte chemokine peptide (MCP-1), inducible nitric oxide synthase (iNOS), and arginase-1 (Arg-1).@*RESULTS@#SBP did not affect the mortality of SHR (P<0.05). SBP significantly reduced the level of elevated blood pressure of SHRs, but the effect was less significantly than that of losartan potassium. SBP decreased urine protein (P<0.01) and the expression levels of IL-1 β, IL-6, TNF-α, and TGF-β in serum. The 22-week-old SHRs showed mild proliferation of glomerular endothelial cells, glomerular ischemic lesions, inflammatory cell infiltration in renal tubular interstitium and arteriosclerosis. Both SBP and losartan potassium had alleviated renal pathological change, and significantly reduced the infiltration of macrophage (P<0.05, P<0.01). SBP and losartan potassium decreased the expressions of TLR4, NF-κB, MCP-1, iNOS, and Arg-1.@*CONCLUSION@#SBP significantly modified the early hypertensive renal injury by reducing inflammation, and the effect was similar to losartan potassium.
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Objective:To explore therapeutic effect and safety of oral musk Baoxin pill in patients with stable angina pectoris (SAP) of coronary heart disease (CHD).Methods:A total of 158 SAP patients of CHD treated in our hos-pital were selected,and were randomly divided into routine treatment group (n＝79) and musk Baoxin pill group (n＝79,received musk Baoxin pill based on routine treatment ),both groups were treated for eight weeks.Therapeutic effect and safety were compared between two groups.Results:After treatment,there was no significant difference in total effective rate of ECG improvement between two groups,P＝0.475.Compared with routine treatment group after treatment,there were significant reductions in onset times of angina pectoris [(1.68 ± 0.43) times/d vs.(1.12 ± 0.37) times/d] and nitroglycerin consumption [(1.65 ± 0.87)/d vs.(1.08 ± 0.47)/d],and significant rise in to-tal effective rate of angina pectoris improvement (81.01% vs.96.20%) in musk Baoxin pill group,P＝0.001 all.No obvious adverse reactions occurred in two groups.Conclusion:Oral musk Baoxin pill based on routine treatment can significantly relieve angina pectoris symptoms and the safety is good in patients with stable angina pectoris of coronary heart disease.
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Objective To observe the clinical effect of Shexiang Baoxin pill,a traditional Chinese medicine,combined with nicorandil on microvascular angina pectoris.Methods Seventy patients with microangiopathic angina diagnosed by coronary angiography admitted to the First Affiliated Hospital of Baotou Medical College from January 2014 to August 2016 were enrolled,and they were divided into a control group and an observation group according to conplete random number table method,35 patients in each group.Both groups were given routine treatment,in the observation group,additionally,nicorandil 5 mg 3 times a day and 2 Shexiang Baoxin pills (22.5 mg per tablet),3 times a day were orally applied,the therapeutic course in both groups being 6 weeks.Before and after treatment,the changes of number of episodes of angina pectoris per week,of the total number of ST-segment depression leads and the minimum voltage amplitude of ST-segment depression in the 12-lead resting electrocardiogram and adverse reaction were observed in the two groups.Results The frequency of angina pectoris after treatment was less than that before treatment,the total number of ST segment depression leads was lower and the minimum voltage amplitude of ST segment depression was decreased compared with those before treatment in the two groups,the degree of change in the observation group being more significant than those in the control goup [angina attack frequency (times):5.2 ± 0.5 vs.9.4 ± 1.6,ST segment depression lead total number:2.3-± 0.2 vs.4.6 ± 2.1,ST segment depression lowest voltage amplitude (my):0.10 ± 0.01 vs.0.31 ± 0.01,all P ＜ 0.05].The adverse effects incidence showed no statistical significant differences between control group and observation group [14.3％ (5/35) vs.22.8％ (8/35),P ＞ 0.05] Conclusions On the basis of conventional medication,Shexiang Baoxin pills combined with nicorandil has obvious therapeutic effect for treatment of microangiopathic angina pectoris.
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Objective To explore the efficacy and safety of Shexiang Baoxin Pill combined with isosorbidedinitrate in the treatment of unstable angina pectoris.Methods 80 cases of UAP patients from Jun.2014 to Jun.2016 were given basic treatment plan,and 40 cases were randomly selected and treated with isosorbidedinitrate as the control group,and the other 40 cases were treated with Shexiang Baoxin Pill combined with isosorbidedinitrate.The incidence of angina pectoris and nitroglycerin dosage,angina pectoris curative effect,electrocardiogram (ECG) curative effect and adverse drug reactions were compared between the two groups.Results Before treatment,daily anginal attacks and nitroglycerin dosage of two groups had no significant difference;after treatment,daily anginal attacks and nitroglycerin dosage between two groups were obviously decreased,the differences of same group before and after treatment was statistically significant (P ＜ 0.05);and the treatment group was significantly less than the control group (P ＜ 0.05).The total effective rate of angina pectoris in the treatment group was higher than that in the control group,the difference was statistically significant (P ＜ 0.05).The total effective rate of ECG in the treatment group was higher than that in the control group,the difference was statistically significant (P ＜ 0.05).Conclusions Shexiang Baoxin Pill combined with isosorbidedinitrate in the treatment of UAP can significantly improve the efficacy,and it's adverse reaction is less.
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Objective To investigate the effects and mechanism of Shexiang Baoxin Pills on insulin resistance (IR) in patients with coronary heart disease.Methods From January 2016 to March 2017,82 patients with coronary heart disease in The Second People's Hospital of Nanyang were selected as the research object.All the patients were divided into observation group and control group with 41 cases in each group according to the principle of random envelope drawing.The control group was given conventional treatment,and the observation group was treated with Shexiang Baoxin Pills on the basis of the treatment of the control group.The observation periods of the two groups were 3 months.Results The total effective rates of the observation group and control group were 97.6％ and 82.9％,respectively,and the observation group was better than that of the control group (P ＜ 0.05).The IR indexes of the observation group and control group after treatment were (1.79 ± 0.45) and (2.44 ± 0.51),respectively,which were significantly lower than before treatment of (4.01 ± 0.44) and (4.00 ± 0.56) (P ＜ 0.05),and the IR index in the observation group after treatment was significantly higher than that in the control group (P ＜ 0.05).The serum CRP levels of the observation group and control group were significantly lower than those before treatment (P ＜ 0.05),and the level of serum CRP in the observation group after treatment was also significantly lower than that in the control group (P ＜ 0.05).Atter treatment,the coronary artery remodeling,no reconstruction and negative reconstruction in the observation group were 4 cases,25 cases and 13 cases,while the control group were 15 cases,10 cases and 16 cases,the difference between the two groups was statistically significant (P ＜ 0.05).Conclusion Shexiang Baoxin Pill can improve the therapeutic effect and improve the IR status in patients with coronary heart disease,and its mechanism may be related to inhibiting the expression of inflammatory factors and improving coronary artery remodeling.
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Objective To investigate the effects and mechanism of Shexiang Baoxin Pills on insulin resistance (IR) in patients with coronary heart disease.Methods From January 2016 to March 2017,82 patients with coronary heart disease in The Second People's Hospital of Nanyang were selected as the research object.All the patients were divided into observation group and control group with 41 cases in each group according to the principle of random envelope drawing.The control group was given conventional treatment,and the observation group was treated with Shexiang Baoxin Pills on the basis of the treatment of the control group.The observation periods of the two groups were 3 months.Results The total effective rates of the observation group and control group were 97.6％ and 82.9％,respectively,and the observation group was better than that of the control group (P ＜ 0.05).The IR indexes of the observation group and control group after treatment were (1.79 ± 0.45) and (2.44 ± 0.51),respectively,which were significantly lower than before treatment of (4.01 ± 0.44) and (4.00 ± 0.56) (P ＜ 0.05),and the IR index in the observation group after treatment was significantly higher than that in the control group (P ＜ 0.05).The serum CRP levels of the observation group and control group were significantly lower than those before treatment (P ＜ 0.05),and the level of serum CRP in the observation group after treatment was also significantly lower than that in the control group (P ＜ 0.05).Atter treatment,the coronary artery remodeling,no reconstruction and negative reconstruction in the observation group were 4 cases,25 cases and 13 cases,while the control group were 15 cases,10 cases and 16 cases,the difference between the two groups was statistically significant (P ＜ 0.05).Conclusion Shexiang Baoxin Pill can improve the therapeutic effect and improve the IR status in patients with coronary heart disease,and its mechanism may be related to inhibiting the expression of inflammatory factors and improving coronary artery remodeling.
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Objective:To observe the effects of Shexiang Baoxin Pill (SBP) on isoprenaline (Iso)-induced changes in myocardial cell volume,shape,and connexin 43 (Cx43) expression.Methods:HgC2 myocardial cells were randomly divided into a control group,a Iso group and a Iso+SBP group.After 72 h of culture,the average surface area of HgC2 cells was measured under phase contrast microscope.Bicinchoninic acid (BCA) protein assay was carried out to determine the concentration of proteins.The survival rate of myocardial cells was measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay,and the Cx43 expression was detected by Western blot.Results:The mean surface area and Cx43 concentration in Iso-treated myocardial cells were increased under the phase contrast microscope (P<0.05).Compared with the Iso group,the mean surface area was decreased,and the Cx43 concentration was reduced in the Iso+SBP group (both P<0.05).Compared with the control group,the Cx43 expression was obviously down-regulated in the H9C2 cells of the Iso group (P<0.05);while compared with the Iso group,the Cx43 expression was obviously up-regulated in the Iso+SBP group (P<0.05).Conclusion:Shexiang Baoxin Pills can prevent Iso-induced myocardial hypertrophy and down-regulate Cx43 expression.
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Shexiang Baoxin pill(SBP) is widely used for treating coronary heart disease in clinic, with ginsenosides as its main effective component. This study was designed to investigate and compare the pharmacokinetic characteristics of five ginsenosides of five compounds after multiple oral administrations, ginseng extract(GE) and SBP in myocardial infarction rats. After intragastric administration to myocardial infarction rats, the plasma samples were analyzed by liquid chromatography tandem triple-quad mass spectrometry. The results showed that Cmax of five compounds in all groups were less than 200 μg•L⁻¹. Tmax of corresponding analytes between groups revealed that ginsenosides in both SBP and GE were absorbed faster than each of the five compounds, indicating that GE and compounds contain components promoting absorption of ginsenosides. The oral administration doses of ginsenosides in SBP were the least in all groups, but with the greatest AUC0-tand AUCINF, which indicated that ginsenosides in SBP had the best absorption in all groups after oral administration to myocardial infarction rats. This study also demonstrated that compound is the best form of traditional Chinese medicine.
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Shexiang Baoxin Pill, which has the functions of activating Yang and benefiting vital energy in resuscitation with aromatics, is widely used in clinic for the treatment of angina pectoris, chest distress, and myocardial infarction induced by myocardial ischemia. Since it is commonly used for the treatment of coronary heart disease and angina pectoris in clinic. The study on its chemical material basis, pharmacology, and systems biology of Shexiang Baoxin Pill have been carried out successively. In this paper, the advance in the modern studies on Shexiang Baoxin Pill has been reviewed.
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Objective: To investigate the effect of Shexiang Baoxin Pill (SBP) on the inhibition of ventricular arrhythmia of rats with acute myocardial infarction. Methods: Ninety male model rats induced by left anterior descending branch ligation were randomly divided into model group, high-dose SBP treatment group, and low-dose SBP treatment group (45.0 and 22.5 mg/kg) equally (n = 30). Every group was further divided into the 1st weekend and the 2nd weekend subunits equally (n = 15). Twenty rats were taken as the Sham operation group which was further divided into the 1st weekend and the 2nd weekend subunits equally (n = 10). The rats in the high-and low-dose groups were respectively treated by ig administration of SBP for 1 or 2 weeks from day 2 after the administration, whereas the rats in the Sham operation group were not given any treatment. At the 1st weekend and the 2nd weekend, programmed electrical stimulus was imposed on the rat hearts to induce the ventricular arrhythmia. Then the left ventricle free wall tissue in the Sham operation group and the border zone myocardium in the rest of rats were preserved. The expression of myocardial IL-18 was determined. Results: Compared to the the Sham operation group, there were higher expression of myocardial IL-18 and the induced ratio of ventricular arrhythmia at 1st weekend and 2nd weekend in the model group (P < 0.01). Compared to the model group, low-dose SBP treatment could reduce the expression of myocardial IL-18 and the induced ratio of ventricular arrhythmia at 2nd weekend (P < 0.05), but there was no difference on the expression of myocardial IL-18 and the induced ratio of ventricular arrhythmia at the 1st weekend. Compared to the the model group, high-dose SBP treatment could reduce the expression of myocardial IL-18 and the induced ratio of ventricular arrhythmia at the 1st weekend and the 2nd weekend (P < 0.01). Compared to the low-dose SBP group, high-dose Shexiang Baoxin Pill treatment could reduce the expression of myocardial IL-18 and the induced ratio of ventricular arrhythmia at the 1st weekend (P < 0.01) and the 2nd weekend (P < 0.05). Conclusion: SBP may reduce the induced rate of ventricular arrhythmia of rats after acute myocardial infarction by inhibiting the over-expression of myocardial IL-18, which is in a dose dependent manner.
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Objective To screen and identify the main pro-angiogenic compounds of Shexiang Baoxin pill ( SBP) presenting in the plasma.Methods The pro-angiogenic effects of SBP and its compounds absorbed into blood were measured by the cell prolif-eration and cell migration assays by xCELLigence .And the cell tube formation and rat aortic ring models were established to evaluate their pro-angiogenic effect.Results SBP(10 -4 ~10 -2 μg/ml), ginsenoside Rg3(1 ~10 μmol/L) and ginsenoside Rh2(1 ~10μmol/L)significantly stimulated human umbilical vein endothelial cells (HUVECs) proliferation, migration and tube-like structures formation at different concentrations (P<0.05).In addition, compared to the control group, only the high concentration group of SBP (10 -2 μg/ml), Rg3(10 μmol/L) and Rh2(10 μmol/L)could induce endothelial cell sprouting from the aortic ring(P<0.05) .Conclusion SBP, ginsenosideRg3 and Rh2 exhibited significantly pro-angiogenic effect in vitro.
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Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill ( SBP) absorbed in blood against hypoxia/reoxygenation injury .Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats (1~3 days old) and were used to build hypoxia/reoxygenation injury model.The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method.Results SBP showed significant protective effect against cardiomyocytes hypoxia /reoxygenation injury atthe concentration of 50 μg/ml.Ginsen-oside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect a -gainst cardiomyocytes hypoxia/reoxygenation injury .Conclusion SBP have the protective activity against cardiomyocytes hypoxia /reoxygenation injury , and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP .This experiment offered basis for further pharmacodynamics and mechanism study of SBP .
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Objective: To study the inhibitory effect of Shexiang Baoxin Pill (SBP) on the immunological liver fibrosis and its possible mechanisms. Methods: Male rats with immunological liver fibrosis were equally divided into control, model, and low-and high-dose (22.5 and 45 mg/kg) SBP groups. Rats in SBP groups were ig given SBP once daily for consecutive 10 weeks. On the week 10, all rats were sacrificed and a part of liver tissues were preserved. The changes of hepatic histopathology were observed using HE staining. Gene expression of vascular endothelial growth factor (VEGF) was observed using RT-PCR. The protein expression of VEGF and α-SMA was detected using immunohistochemistry, and MDA content, SOD activity, and the area of collagen fiber in the liver before and after treatments were measured. Results: Compared to the model control, low-and high-dose SBP groups could relieve the degree of hepatic fibrosis (P < 0.01, 0.05), reduce the expression of hepatic VEGF mRNA, VEGF, α-SMA (P < 0.01), obviously reduce MDA content and the area of collagen fiber (P < 0.05, 0.01), and increase the activity of SOD (P < 0.05, 0.01). All the effects were dose-dependent. Conclusion: SBP may decrease the expression of hepatic VEGF and reduce the level of oxidative stress. The inhibitory effect may depend on its dose, which may be based on inhibiting the activation of hepatic stellate cells.
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ObjectiveTo evaluate the clinical effect and safety of coronary artery disease (CAD) treated with Shexiang-baoxin pill and Complex Danshen drop pill.MethodsArticles of randomized controlled trials (RCTs) about the treatment of CAD with Shexiang-baoxin pill and Complex Danshen drop pill were retrieved and the quality of these studies were analyzed by RevMan 5.1.6 software with Meta analysis.Results Seven studies involving 603 patients were included.The results of meta-analysis showed that the experimental group was better in ameliorating angina symptoms of angina pectoris patients [RR=1.33,95％CI (1.21,1.45),P＜0.01 ] and ECG [RR=1.55,95％CI (1.34,1.79),P＜0.01 ] than the control group.Adverse reaction rate of the experimental group and the control group was 6.85％ and 14.38％ respectively,the difference was not statistically significant (P＞0.05).ConclusionThe existing researches demonstrated that Shexiang-baoxin pill was better in treating CAD angina pectoris than Complex Danshen drop pill.
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AIM:To observe the improvement in the endothelial function of patients with long-term use of Shexiang Baoxin Pill(Moschus,Ginseng extract,Calculus bovis,Cortex cinnamomi,Styrax,Venenum bufonis,and Borneolum Syntheticum)(SXBXP).METHODS:Eighty patients with CHD were randomly assigned into the SXBXP group and the control group.Two groups were controlled with the usual treatment.SXBXP 2 pills,po.tid.were taken in the SXBXP group and continued for at least 6 months.The ultrasound assessment of endothelial-dependent flow-mediated vasodilation(FMD)of the brachial artery was applied in the 3rd,6th and 18th and simultaneous determination of nirtogen oxides(NO),nitricoxide synthase(NOS),superoxide dismutase(SOD),endothelin(ET)was made.RESULTS:Follow-up checkups revealed that the level of FMD and SOD in the 3rd month,the level of NO,NOS and ET in the 6th month in SXBXP group were better than those in the control group at the significant level(P＜0.05).Patient keeping on taking SXBXP up to the 18th month,the obvious improvement in the level of FMD,NO,NOS and SOD was showed(P＜0.05).CONCLUSION:Administration of SXBXP could improve endothelial function in the CHD patients significantly.
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Objective:To explore the expression of LOX-1(lectin like oxidizeol low density lipoprotein receptor-1,LOX-1) in aorta of atherosclerotic rabbits and modulating the effect of fluvastatin and Shexiang baoxin pill on the gene and protein expression of LOX-1. Methods:Tirty-two New Zealand white rabbits were randomly divided into four groups: hypercholesterol diet group (fed mth high cholesterol diet),hypercholesterol diet plus Fhevastatin group(fed with high cholesterol diet plus Fhe vastain 25mgl kgld).hypercholesterol diet plus shexiang Bao xin Pill group (fed with high cholesterol diet plus shegxiang Baoxinpill 25mgl kgld).normal diet gwup(fed mith regnlar chow),the protein expression of LOX-1 in aorta of rabbits were examined by immunohistochemsthy,the gene expresswin of LOX-1 in aorte of rabbits were examined by R7-PCK. Results:hypercholesterol diet group(fed with high cholesterol diet),hypercholesterol diet plus fluvastatin group(fed with high cholesterol diet plus Fluvastatin 25mg/kg?d), hypercholesterol diet plus Shexiang baoxin pill group(fed with high cholesterol diet plus Shexiang Baoxin Pill 25mg/kg?d),normal diet group(fed with regular chow),the protein expression of LOX-1 in aorta of rabbits were examined by immunohistochemistry,the gene expression of LOX-1 in aorta of rabbits were examined by RT-PCR. Conclusion:LOX-1 was expressed in rabbit aorta of hypercholesterol diet group and two treatment groups, expression of LOX-1 in two treatment groups both significantly reduced compared with hypercholesterol diet group(P
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AIM: To study the dissolution of ginsenoside Rb1,cinnamic acid and borneol in traditional Chinese medicine Shexiang Baoxin Pill(Moschus,extract of Radix et Rhizoma ginseng,Calculus Bovis artifactus,Cortex Cinnamomi,Styrax,Venenum Bufonis and Borneolum syntheticum). METHODS: The in vitro dissolution of active components in Shexiang Baoxin Pill was performed with paddle method. HPLC was used for determination of ginsenoside Rb1 and cinnamic acid,and GC for borneol. RESULTS: The analytic method above was easy to carry out,the requirement of the specificity,accuracy,precision and stability could meet the needs of experiment well; the dissolution time T50 and Td of ginsenoside Rb1,cinnamic acid and borneol were 30. 90,33. 79 min,29. 73, 37. 11 min and 42. 38,48. 51 min respectively. CONCLUSION: The dissolution rate of three active components from Shexiang Baoxin Pills was fast and the analytic methods established in this study could be used to evaluate the quality of the preparation.
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AIM: To investigate the effect of Shexiang Baoxin Pill(SXBXP) on endothelial in stable angina pectoris patients and its underlying mechanisms. METHODS: Sixty-eight stable angina pectoris patients were randomly divided into two groups,the conventional group(34cases),SXBXP treatment group(34cases).The conventional group was treated with standard treatment,and SXBXP group was treated with standard treatment and SXBXP.FMD,NO,ET,TXB_2,6-Keto-PGF-1a were determined before and after three months treatment. RESULTS: FMD,NO,6-Keto-PGF-1a of SXBXP group were (9.35%?0.78%)、(77.25?6.36)?mmol/L、(93.87?(10.28))?/(ng/L) after treatment,ET、TXB_2 were(81.15?5.43) pg/mL、(43.02?4.19)?/(ng/L).There were significant improvement as compared with in SXBXP group before treatment and in conventional group after treatment(P